Acanthodactylus Wiegmann, 1834 is one of the most diverse and widespread lizard genus in the Palearctic realm. Here, we describe a new species, - Acanthodactylus ilgazi sp. nov. - from the Anatolian Peninsula. This new species ranges approximately 250 km north from the closest population of this genus in Turkey. Compared to other fringe-fingered lizards, the new species is phylogenetically close to A. robustus, A. tristrami and A. orientalis but it has some distinct morphological characteristics: reddish coloration under the tail, a sharp white or grayish stripe in the middle of the dorsum, and four plates in a row on the 4th finger. Moreover, phylogenetic molecular data, based on cyt b gene fragment, verifies that the new species is phylogenetically a member of the tristrami species group with 13.03%, 17.35% and 20.56 genetic distance respectively from A. orientalis, A. tristrami and A. robustus. Lastly, the known range of this species, located in Yazıhan, Malatya in Eastern Anatolia, is restricted by a dam, thus habitat loss endangers its continuity. Therefore, the conservation status of this species should be assessed immediately.