Document Type : Original Article

Authors

1 Ege University

2 Girne American University

Abstract

Sharks and rays, besides their economic importance, are ecologically important organisms with a diverse group. After a pregnant Squatina sp. was captured as by-catch, Akyol et al. (2015) published this species as Squatina squatina while the COI and 16S rDNA analysis demonstrated that species is S. aculeata. This complexity revealed that utilizing morphologic identification solely might not be enough to distinguish of these two Squatina species accurately. We aim to compare morphologic and molecular techniques during species identification of critically endangered S. squatina and S. aculeata. Two different gene regions were used for molecular identification of 3 Squatina specimen obtained from the Aegean coasts of Turkey. Sequence analysis of two gene regions were conducted after PCR analysis. Aligned data set was used for creating phylogenetic trees.

The results demonstrated that previously identified S. squatina specimen revealed as S. aculeata after molecular analysis. Two other specimens which were morphologically identified as S. squatina demonstrated the same results both with molecular and morphological analysis. Our results suggest that, adopting morphological identification as the only tool is not enough to accurately determine the Squatina species; both morphological and molecular tools should be used for taxonomical identification of shark species, especially for the endangered ones, to assure their conservation status.

Keywords

Main Subjects