DNA barcoding of the Luristan newt (Neurergus kaiseri) in south-western Iran


  • Hadi Khoshnamvand Department of Natural Resources, Isfahan University of Technology, Isfahan, IRAN
  • Mansoureh Malekian Department of Natural Resources, Isfahan University of Technology, Isfahan, IRAN
  • Yazdan Keivani Department of Natural Resources, Isfahan University of Technology, Isfahan, IRAN
  • Forough Goudarzi Department of Natural Resources, Isfahan University of Technology, Isfahan, IRAN




Amphibian, COI, habitat features, phylogeny


The Luristan newt (Neurerguskaiseri) is an endemic and vulnerable species to the southern Zagros Mountains of Iran, inhabiting streams and ponds in open woodlands, dominated by oak tree (Quercus brantii). In the current study, 20 specimens of the Luristan newt were collected and sequenced for a 651bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (CO1). Sequence divergence values varied from 0.0% to 2.8%. The phylogenetic analysis revealed the existence of two distinct clades and a clear divergence between Northern and Southern populations. The genetic variation among the two clades was significant (FST = 0.95, < 0.001). Five haplotypes were observed among N. kaiseri sequences, of which two haplotypes were found in the Northern populations and the other three haplotypes in the Southern populations. None of the haplotypes was shared between the two clades. The distinction of Northern and Southern populations may be due to the rough topography of its habitats and the low dispersal ability of the Luristan newt.


Arbogast B.S., Kenagy G.J. 2001. Comparative phylogeography as an integrative approach to historical biogeography. Journal of Biogeography 28: 819-825.

Bandelt H. J., Forster P., Rohl A. 1999. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution 16: 37-48.

Chambers E. A., Hebert P. D. N. 2016. Assessing DNA barcodes for species identification in North American reptiles and amphibians in natural history collections. Plos One 11: e0154363.

CITES 2010. Convention on International Trade in Endangered Species of Wild Fauna and Flora, Considerations for Proposals of Amendment of Appendices I and II, CoP15 Prop.14, Doha, Qatar. http://www.cites.org/eng/cop/15/prop/E-15%20Prop-14.pdf (accessed 2.4.16).

Estupiñán R. A., Ferrari S. F., Gonçalves E. C., Barbosa M. S. R., Vallinoto M., Schneider M.P.C. 2016. Evaluating the diversity of Neotropical anurans using DNA barcodes. ZooKeys: 89-106.

Excoffier L., Lischer H. L. 2010. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10: 564-567.

Farasat H., Akmali V., Sharifi M. 2016. Population genetic structure of the endangered Kaiser’s mountain newt, Neurergus kaiseri (Amphibia: Salamandridae). Plos One 11: e0149596.

Farasat H., Sharifi M. 2015. Ageing and growth of the endangered Kaiser's mountain newt, Neurergus kaiseri (Caudata: Salamandridae), in the southern Zagros range, Iran. Journal of Herpetology 50: 120-125.

Fouquet A., Gilles A., Vences M., Marty C., Blanc M., Gemmell N. J. 2007. Underestimation of species richness in neotropical frogs revealed by mtDNA analyses. Plos One 2: e1109.

Frankham R., Ballou D., Briscoe D. A. 2004. A Primer of Conservation Genetics, Cambridge University Press, Cambridge.

Friedheim S. 2016. Comparison of species identification methods: DNA barcoding versus morphological taxonomy. Mānoa Horizons 1: 74-86.

Hebert P., Gregory T. R. 2005. The promise Of DNA barcoding for taxonomy. Systematic Biology 54.5: 852-859.

Hebert P. D. N., deWaard J.R. 2010. DNA barcodes for 1/1000 of the animal kingdom. Biology Letters 6: 359-362.

Hebert P. D. N., Ratnasingham S., de Waard J. R. 2003. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B 270: S96-S99.

IUCN 2018. The IUCN Red List of Threatened Species. Version 2018-1. available at www.iucnredlist.org. accessed on 17 July 2018.

Jing C., Hong-Man C., Jun-Xiao Y., Jie-Qiong J., Ke J., Zhi-Yong Y., Murphy R., Zhang Y. 2012. Universal COI primers for DNA barcoding amphibians. Molecular Ecology Resources 12.

Mobaraki A., Amiri M., Alvandi R., Ebrahim Tehrani M., Zarin Kia H., Khoshnamvand A., Bali A., Forozanfar E., Browne R. 2014. Conservation reassessment of the critically endangered, Luristan newt Neurergus kaiseri (Schmidt 1952) in Iran. Amphbian & Reptile Conservation 9: 16- 25.

Mobaraki A., Safaei Mahroo B., Torki F., Sharifi M., Rastegar-Pouyani N., Kuzmin S., Anderson S., Papenfuss T., Dibadj P. 2016. Neurergus kaiseri, IUCN Red List of Threatened Species. Version 3.1. Available at: http://dx.doi.org/10.2305/IUCN.UK. 2016-3.RLTS.T59450A49436271.en. Accessed:14 August 2018.

Pereyra M. O., Baldo D., Blotto B. L., Iglesias P. P., Thomé M. T. C., Haddad C. F. B., Barrio-Amorós C., Ibañez R., Faivovich J. 2016. Phylogenetic relationships of toads of the Rhinella granulosa group (Anura: Bufonidae): A molecular perspective with comments on hybridization and introgression. Cladistics 32: 36-53.

Posada D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25: 1253-1256.

Simons A.M. 2002. The continuity of microevolution and macroevolution. Journal of Evolutionary Biology 15: 688-701.

Sharifi M., Afroosheh M. 2014. Studying migratory activity and home range of adult Neurergus microspilotus (Nesterov, 1916) in the Kavat Stream, western Iran, using photographic identification (Caudata: Salamandridae). Herpetozoa 27: 77-82.

Sharifi M., Farasat H., Barani-Beiranvand H., Vaissi S., Foroozanfar E. 2013. Notes on the distribution and abundance of the endangered Kaiser’s Mountain Newt Neurergus kaiseri (Caudata: Salamandridae) in South-western Iran. Herpetological Conservation & Biology 8: 724-731.

Smith M.A., Poyarkov N., Hebert P. 2008. DNA barcoding: CO1 DNA barcoding amphibians: take the chance, meet the challenge. Molecular Ecology Resources 8: 235-246.

Sork, V.L., Waits, L. 2010: Contributions of landscape genetics: approaches, insights, and future potential. Molecular Ecology 19: 3489-3495.

Stuart B. L., Inger R. F., Voris H. K. 2006. High level of cryptic species diversity revealed by sympatric lineages of Southeast Asian forest frogs. Biology Letters 2: 470-474.

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30: 2725-2729.

Valentini A., Pompanon F., Taberlet P. 2008. DNA barcoding for ecologists. Trends in Ecology and Evolution 24: 110-117.

Vences M., Thomas M., Bonett R. M., Vieites D. R. 2005. Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philosophical Transactions of the Royal Society B: Biological Sciences 360: 1859-1868.

Xia Y., Gu H. F., Peng R., Chen Q., Zheng Y. C., Murphy R. W., Zeng X. M. 2012. COI is better than 16S rRNA for DNA barcoding Asiatic salamanders. Molecular Ecology Resources 12: 48-56.




How to Cite

Khoshnamvand, H., Malekian, M., Keivani, Y., & Goudarzi, F. (2019). DNA barcoding of the Luristan newt (Neurergus kaiseri) in south-western Iran. Journal of Wildlife and Biodiversity, 3(2), 11–17. https://doi.org/10.22120/jwb.2019.34933