Isolation and identification of bacteria with cellulolytic activity from the microflora of animal juice
DOI:
https://doi.org/10.5281/zenodo.8308108Keywords:
Bacillus subtilis, microflora, gastric juice, animal husbandry, celluloseAbstract
According to current modern concepts, cellulose complexes produced by various microorganisms are polyfermentative systems composed of enzymes that differ in molecular structure and the way they act on cellulose. Microbiological processing is one of the most economical, promising and ecologically safe ways of processing cellulose raw materials. In the study, 27 spore-forming microorganism isolates were isolated from goat stomach juice, goat stomach, rabbit stomach juice, chicken stomach, bark beetle (Dendroctonus ponderosae) and termites (Coptotermes formosanus). Morphological characteristics of bacterial isolates were studied and identified using matrix laser desorption/ionization mass spectrometry (MALDI-TOF) method and genetic method. Based on the primary screening, the cellulolytic activity of the isolated bacteria was evaluated by reducing the mass of #10 100% cotton thread from 0.05 g to 0.035 g after 96 hours. Freshly obtained goat gastric juice was used as a control. The cellulolytic activity of the bacterial strains was determined by the application of Congo red to the agar medium. 1-Bacillus isolated from the termite (Coptotermes formosanus) when cellulolytic activity was carried out according to the modified method of Mendels and Weber. sp. and isolated from goat gastric juice 4- B. subtilis sp. the highest activity of 0.233 and 0.193 TslS/cm3 was determined in the strains. Also 1-Bacillus. sp. and 2- Bacillus. sp. bacterial strains produce up to 0.34 mg/ml of protein and the highest activity was isolated from goat gastric juice 3- B. subtilis sp. strain and was 0.38 mg/ml. In our research, we determined the cellulolytic activity of Bacillus strains isolated from the bark beetle (Dendroctonus ponderosae) and termites (Coptotermes formosanus), which feed on roughage consisting of cellulose. serves to create.
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