Preparation of Polyclonal Antiserum for potato X virus
DOI:
https://doi.org/10.5281/zenodo.10206960Keywords:
Potato Х virus, antigen, isotonic solution, double-immunodiffusion, polyclonal antiserumAbstract
Recently, as a result of the development of agriculture, and the expansion of the international export of seeds and seedlings, pathogenic viruses that infect potatoes have spread to other regions, and changes in viruses under the influence of various climatic factors have led to the emergence of strong pathogenic isolates. The preservation of potato viruses in potato nodules and other plant organs creates several difficulties in their diagnosis. For this, it is advisable to use highly sensitive diagnostic methods. In this work, the results of the research on the preparation of polyclonal serum, which is the basis for obtaining antibodies necessary for the immunodiagnosis of potato X virus (PXV), are given. In this case, an isotonic solution of NaCl or cycloferon together with a purified virus antigen was injected between the muscles of the biceps and hind legs of rabbits. The delivered antigen dose was increased by the same amount for both rabbits. Before reimmunization, the titer of sera from the rabbit injected with cyclotron was 1:64, the titer from the rabbit injected with an isotonic solution of sodium chloride was 1:32, and after reimmunization, the titer of the serum isolated from the first rabbit decreased to 1:32, and from the rabbit injected with NaCl the resulting serum was found to be elevated to a titer of 1:256. The prepared serum was used in the diagnosis of PXV and its amount in plant tissue was determined.
References
Abdenaceur, R., Farida, B., Mourad, D. (2022). Effective biofertilizer Trichoderma spp. isolates with enzymatic activity and metabolites enhancing plant growth. Int Microbiol 25, 817–829. https://doi.org/10.1007/s10123-022-00263-8
Ahmed, M. S., Khalid, A. A., Adel, A.R., Hossam, S. E., Sherif, M. E. (2019). Production of polyclonal antibodies to some potato viruses using synthetic peptides. Fresenius Environmental Bulletin, 28(6), 4870-4877.
Amanov, B., Abdiev, F., Muminov, K., Shavkiev, J., Mamedova, F. (2020). Valuable economic indicators among hybrids of peruvian cotton genotypes. Plant Cell Biotechnology and Molecular Biology, 21(67-68), 35–46
Anisimov, B.V., Belov, G.L., Vahisev, Y.A., Elansky. S.N. (2009). Protection from potato pest’s diseases and weeds. Kartofelevo, 272.
Berzina, A.G., Gamaleya, N.B. Kapanadze, G.D. (2013). Methodical approaches to obtaining antispecies antisera for the purpose of their use in immunopharmacological studies. Biomedicine, 2, 95–102.
Bikash, M., Alok, K., Pooja, R., Rakesh, K. (2012). Complete Genome Sequence, Phylogenetic Relationships and Molecular Diagnosis of an Indian Isolate of Potato Virus X. Journal of Phytopathology, 160(1), 1-5. https://doi.org/10.1111/j.1439-0434.2011.01848.x.
Čeřovská, N., Moravec, T., Rosecká, P., Dědič, P., Filigarová, M. (2003). Production of polyclonal antibodies to a recombinant coat protein of potato mop-top virus. J.Phytophatol. 151, 1–6.
Cong, Q.Q., Wang, Y., Liu, J. (2019). Evaluation of Potato virus X mild mutants for cross protection against severe infection in China. Virol J, 16, 36. https://doi.org/10.1186/s12985-019-1143-7
Egorov, A. M., Osipov, A. P., Dzantnev, B. B., Gavrilova, E. M. (1991). Theory and practice of enzyme immunoassay. Higher school, 288.
Eskendirova, S.Z., Sarina, N.I., Manat, E. (2014). Preparation and immunochemical characterization of monoclonal antibodies to potato X-virus. Biotechnology. Theory and practice. 4, 35-43.
Fayziev, V. B. (2019). Isolation of potato X-virus spread in Uzbekistan, study of its characteristics and its diagnosis. Doc. diss. Tashkent. 230.
Fayziev, V., D. Jovlieva, Z., Sobirova, N., Shonazarova, G., Suyunova, N., Berdikulova, E. (2023. Identification of Potato virus X in Uzabekistan and its biological and molecular genetic characterization”. Eur. Chem. Bull. 12 (4), 18254-18265.
Fayziev, V., Jovlieva, D., Juraeva, U., Shavkiev, J., Eshboev, F. (2020). Effects of PVXN-UZ 915 necrotic isolate of Potato virus X on amount of pigments of Datura stramonium leaves. Journal of Critical Reviews, 7(9), p 400–403.
Gnutova, R. V., (1987). The role of the immunogenicity of phytoviruses in receiving immunodiagnosticums. Published online.
Isakov, D.V., and Isakov V.A. (2015). Cycloferon: mechanism of action, and new prospects for application in clinical practice. Klin.med. 93 (9): 46—51.
Isakov, V.A., and Isakov, D. V. (2014). Immunomodulators in Therapy of Respiratory Infections. Antibiotics and chemotherapy, 59(11-12), 27-34
Kerstin, U., Verena, S., Sergei, V., Rainer, F., Ulrich, C. (2010). Immunogenic properties of chimeric potato virus X particles displaying the Hepatitis C virus hypervariable region I peptide R9. Journal of Virological Methods 166, 12–20.
Kharitonova L.A., Israfilova O.E. (2018). Experience of cycloferon therapy in the complex therapy of recurrent respiratory infections in children. Ros Vestn Perinatol and Pediatr, 63:(3), 98–104.
Khusanov, T. S., Kadirova, Z. N., Davronov, K. S., Akhmedova Z. R. (2020). Analysis Of Lysimetric Parameters Of The Effect Of Chemical And Biological Drugs Against Alfalfa Mosaic Virus. The American Journal Of Agriculture And Biomedical Engineering. 25-29.
Muminov, K., Amanov, B., Buronov, A., Tursunova, N., Umirova, L. (2023). Analysis of yield and fiber quality traits in intraspecific and interspecific hybrids of cotton. Sabrao Journal of Breeding and Genetics, 55(2), 453–462.
Ramazonov, B.R., Mutalov, K.A., Fayziev, V.B., Koraev S.B. (2020). Morphogenetic characteristics and biological activity of Takyr and meadow soils of the Republic of Karakalpakstan (On the example of soils of chimbay district) morphogenetic characteristics and biological activity of Takyr and Meadow soils of the Republic of Karakalpakstan (on the example of soils of Chimbay District). Journal of Critical Reviews, 7(5), 243–249.
Shavkiev, J., Karimov, H.Kh., Turaeva, B.I., Nazirbekov, M.Kh., Khamidova, K.M. (2022). Some volatile metabolites produced by the antifungal-trichoderma asperellum uz-a4 micromycete. International Journal of Phytopathology, 11(3), 239–25.
Sobirova, Z.S., Fayziev, V.B., Abduraimova K.I. (2020). Effect of the virus of the yellow dwarf corn mosaic growth and development of varieties of corn in various phases. Journal of Advanced Research in Dynamical and Control Systems, 12(6 Special Issue), 602–606.
Topkaya, Ş. (2020) Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province. Journal of Agricultural Faculty of Gaziosmanpasa University. 37 (1), 53-59.
Turaeva, B., Kutlieva, G., Kamolova, K., Zukhritdinova, N. (2023). Isolation and identification of bacteria with cellulolytic activity from the microflora of animal juice. Journal of Wildlife and Biodiversity, 7(4), 241–264.
Turaeva, B.I., Soliev, A.B., Karimov, H.K., Khamidova, K.M., Zuxritdinova, N.Y. (2021). Disease causing phytopathogenic micromycetes in citrus in Uzbekistan. Pakistan Journal of Phytopathology, 33(2), 383–393.
Zhang, Yu et al. (2020). Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers. Journal of Integrative Agriculture, 19(5), 1283–1291.
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