First evidence of the genetic structure of Neurergus strauchii munzurensis
Keywords:
Anatolian newt, Phylogeny, Salamandridae, UrodelaAbstract
Until 2016, two subspecies of Neurergus strauchii were identified; at this time, one more subspecies was described from Tunceli as N. s. munzurensis. However, this study mainly focused on morphological characters. We aim to test of Neurergus species, including N. s. munzurensis taxa by phylogenetic analysis, using partial base sequences of 12S rRNA and D-loop genes. For this purpose, we reconstructed Bayesian Inference and Maximum Likelihood trees with other Neurergus samples. According to the results, phylogenetic tree topologies demonstrate that N. crocatus was separated from the N. strauchii-N. barani lineage and the N. strauchii group have shown monophyly. Also, N. s. munzurensis showed higher genetic distances with N. barani (1.1%) and N. s. strauchii (0.8%) while genetic distances were only determined as 0.5% within N. barani and N. s. strauchii. N. s. munzurensis was found to be a genetically distinct taxon, following its previous morphological description. This study obtained the first evidence on better explaining the taxonomic status of N. s. munzurensis.
References
Akaike, H. (1974). A new look at the statistical model identification. IEEE Transaction on Automatic Control, 19(6), 716–723.
Darriba, D., Taboada, G.L., Doallo, R., & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772. * https://doi.org/10.1038/nmeth.2109
Doyle, J.J., & Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12(13), 39–40.
Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.
Ficetola, G.F., Falaschi, M., Bonardi, A., Padoa-Schioppa, E., & Sindaco, R. (2018). Biogeographical structure and endemism pattern in reptiles of the Western Palearctic. Progress in Physical Geography: Earth and Environment, 42 (2), 220–236. * https://doi.org/10.1177/0309133318765
Hendrix, R., Fleck, J., Schneider, W., Schneider, C., Geller, D., Avcı, A., Olgun, K., & Steinfartz, S. (2014). First comprehensive insights into nuclear and mitochondrial DNA based population structure of Near East mountain brook newts (Salamandridae: genus Neurergus) suggest the resurrection of Neurergus derjugini. Amphibia-Reptilia, 35: 173–187. * https://doi.org/10.1163/15685381-00002939
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35, 1547–1549. * https://doi.org/10.1093/molbev/msy096
Kurnaz, M. (2020). Species list of Amphibians and Reptiles from Turkey. Journal of Animal Diversity, 2(4), 10–32. * https://doi.org/10.52547/JAD.2020.2.4.2
Kurnaz, M., & Şahin, M.K. (2021). A contribution to the biogeography and taxonomy of two Anatolian mountain brook newts, Neurergus barani and N. strauchii (Amphibia: Salamandridae) using ecological niche modeling. Turkish Journal of Zoology, 45, 54–64. * https://doi.org/10.3906/zoo-2007-37
Leaché, A.D., & Reeder, T.W. (2002): Molecular systematics of the eastern fence lizard (Sceloporus undulatus): a comparison of parsimony, likelihood, and Bayesian approaches. Systematic Biology, 51, 44–68. * https://doi.org/10.1080/106351502753475871
Olgun, K., Avcı, A., Bozkurt, E., Üzüm, N., Olgun, H., & Ilgaz, Ç. (2016). A new subspecies of Anatolia newt, Neurergus strauchii (Steindachner, 1887), from Tunceli, Eastern Turkey. Russian Journal of Herpetology, 23, 271–277. * https://doi.org/10.30906/1026-2296-2016-23-4-271-277
Özdemir, N., Üzüm, N., Avci, A., & Olgun, K. (2009). Phylogeny of Neurergus crocatus and Neurergus strauchii in Turkey based on morphological and molecular data. Herpetologica, 65, 280–291. * https://www.jstor.org/stable/25621901
Pasmans, F., Bogaerts, S., Woeltjes, T., & Carranza, S. (2006). Biogeography of Neurergus strauchii barani Öz, 1994 and N. s. strauchii (Steindachner, 1887) (Amphibia: Salamandridae) assessed using morphological and molecular data. Amphibia-Reptilia, 27, 281–288. * https://doi.org/10.1163/156853806777239878
Rancilhac, L., Goudarzi, F., Gehara, M., Hemami, M.-R., Elmer, K. R., Vences, M., & Steinfarz, S. (2019). Phylogeny and species delimitation of near Eastern Neurergus newts (Salamandridae) based on genome wide RADseq data analysis. Molecular Phylogenetics and Evolution, 133, 189–197. * https://doi.org/10.1016/j.ympev.2019.01.003
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Sucharda, M.A., & Huelsenbeck, J.P. (2012). MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology, 61(3), 539–542. * https://doi.org/10.1093/sysbio/sys029
Vaissi, S. (2022). The role of climatic niche divergence in the speciation of the genus Neurergus: An inter-and intraspecific survey. Evolutionary Ecology, 36, 389–407. * https://doi.org/10.1007/s10682-022-10172-x
Zang, P., Papenfuss, T.J., Wake, M.H., Qu, L., & Wake, D.B. (2008). Phylogeny and biogeography of the family Salamandridae (Amphibia: Caudata) inferred from complete mitochondrial genomes. Molecular Phylogenetics and Evolution, 49, 586–597. * https://doi.org/10.1016/j.ympev.2008.08.020
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Journal of Wildlife and Biodiversity
This work is licensed under a Creative Commons Attribution 4.0 International License.
JWB applies the 